產(chǎn)品介紹

BD Pharmingen FITC小鼠抗人CD3 555339

克隆 HIT3a (RUO)，BD Pharmingen™ FITC Mouse Anti-Human CD3

品牌：BD Pharmingen™

別名：CD3E;T3E;TCRE;CD 3;CD-3 的;CD3 的;CD3-ε;916

反應(yīng)：人體 （QC 測(cè)試）

同種型：小鼠 IgG2a，k

應(yīng)用：流式細(xì)胞術(shù)（常規(guī)測(cè)試）

每次測(cè)試的量：20 微升

存儲(chǔ)緩沖區(qū)：含有 BSA、蛋白質(zhì)穩(wěn)定劑和 ≤0.09% sodium azide的緩沖液。

BD Pharmingen FITC小鼠抗人CD3 555339

準(zhǔn)備和存儲(chǔ)

在 4°C 下原液保存，避免長(zhǎng)時(shí)間光照。不要凍結(jié)。通過(guò)親和層析從組織培養(yǎng)上清液或腹水中純化單克隆抗體。在優(yōu)良條件下將抗體與 FITC 偶聯(lián)，并去除未反應(yīng)的 FITC。

HIT3a

The HIT3a monoclonal antibody specifically binds to the human CD3ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).